bromination of cholesterol mechanism

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M. et al. 56, 319330 (2015). PI(4,5)P2 is translocated by ABCA1 to the cell surface where it mediates apolipoprotein A1 binding and nascent HDL assembly. Zelcer, N., Hong, C., Boyadjian, R. & Tontonoz, P. LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor. Control of ACAT2 liver expression by HNF4 lesson from MODY1 patients. The mechanism appears to be due to the formation of sudanophilic bromo-derivatives of cholesterol and to the retention of certain other lipids, notably phosphatidyl choline and free fatty acids, during staining. Publication: Journal of Chemical Education. Pramfalk, C., Davis, M. A., Eriksson, M., Rudel, L. L. & Parini, P. Control of ACAT2 liver expression by HNF1. J. Balasubramaniyan, N., Ananthanarayanan, M. & Suchy, F. J. Cell. laboratory is supported by grants from the National Natural Science Foundation of China (91753204, 31600651, 31690102, 91857000 and 31771568) and the Ministry of Science and Technology of China (2016YFA0500100 and 2018YFA0800700). ACAT2 and ABCG5/G8 are both required for efficient cholesterol absorption in mice: evidence from thoracic lymph duct cannulation. Google Scholar. Quazi, F. & Molday, R. S. Differential phospholipid substrates and directional transport by ATP-binding cassette proteins ABCA1, ABCA7, and ABCA4 and disease-causing mutants. Yu, L. et al. & Shimano, H. Regulation of sterol regulatory element binding proteins in livers of fasted and refed mice. Internet Explorer). Cholesteryl ester accumulation induced by PTEN loss and PI3K/AKT activation underlies human prostate cancer aggressiveness. J. Biol. Cases, S. et al. CellBiol. Xiao, X. et al. The structure of the NPC1L1 N-terminal domain in a closed conformation. 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New insights into cellular cholesterol acquisition: promoter analysis of human HMGCR and SQLE, two key control enzymes in cholesterol synthesis. Laden, B. P., Tang, Y. Mutations in NPC1 cause 95%of NPC cases. The larger E a c t is associated with greater product selectivity, since the tertiary . 8, 512521 (2008). Yang, T. et al. Arterioscler. A plant sterol with a chemical structure very similar to that of cholesterol. Morris, L. L., Hartman, I. Najafi-Shoushtari, S. H. et al. Sato, R., Goldstein, J. L. & Brown, M. S. Replacement of serine-871 of hamster 3-hydroxy-3-methylglutaryl-CoA reductase prevents phosphorylation by AMP-activated kinase and blocks inhibition of sterol synthesis induced by ATP depletion. Biochem. Davis, H. R. et al. Phillips, M. C. Molecular mechanisms of cellular cholesterol efflux. Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice. Vaughan, A. M. & Oram, J. F. ABCG1 redistributes cell cholesterol to domains removable by high density lipoprotein but not by lipid-depleted apolipoproteins. eLife 5, e21635 (2016). Thekey factors governing these pathways and the major mechanisms by which they respond tovarying sterol levels are described. J. Clin. 25, 11341141 (2008). IDOL stimulates clathrin-independent endocytosis and multivesicular body-mediated lysosomal degradation of the low-density lipoprotein receptor. PubMed J. Biol. J. J. Biol. Biochem. Cell Metab. Luo, J., Jiang, L. Y., Yang, H. Y. J. Naeli, P., Azad, F. M., Malakootian, M., Seidah, N. G. & Mowla, S. J. Post-transcriptional regulation of PCSK9 by miR-191, miR-222, and miR-224. Ezetimibe blocks the internalization of NPC1L1 and cholesterol in mouse small intestine. Cao, J. et al. Nat Rev Mol Cell Biol 21, 225245 (2020). Back, S. S. et al. Sci. 284, 2248122490 (2009). Cell 14, 24472460 (2003). & Schluter, K. D. Physiological and therapeutic regulation of PCSK9 activity in cardiovascular disease. J. Biol. The core components of the Skp, Cullin, F-box (SCF) complex include the scaffold protein Cul1, the RING-finger protein RBX1/ROC1 and the adaptor protein Skp1. J. Biol. Mol. Preliminary bromination (with bromine water) increases the intensity of staining of tissue lipids with Sudan Black and certain other dyes. Biochem. Chem. Why does acetate ion not attack the intermediate bromonium ion to give the 5-acetoxy-6-bromo compound in the bromination of cholesterol? 18, 361374 (2017). Nguyen, A. D., McDonald, J. G., Bruick, R. K. & DeBose-Boyd, R. A. Hypoxia stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme a reductase through accumulation of lanosterol and hypoxia-inducible factor-mediated induction of Insigs. Comparative genome analysis of potential regulatory elements in the ABCG5ABCG8 gene cluster. The E3 ubiquitin ligase MARCH6 degrades squalene monooxygenase and affects 3-hydroxy-3-methyl-glutaryl coenzyme a reductase and the cholesterol synthesis pathway. Circ. Open Access Accumulation of dietary cholesterol in sitosterolemia caused by mutations in adjacent ABC transporters. 20, 85101 (2019). The deubiquitylase USP2 regulates the LDLR pathway by counteracting the E3-ubiquitin ligase IDOL. Horton, J. D. et al. & Ye, J. Sterol-regulated degradation of Insig-1 mediated by the membrane-bound ubiquitin ligase gp78. Lee, J. N., Song, B., DeBose-Boyd, R. A. J. Biol. Nagata, K. O., Nakada, C., Kasai, R. S., Kusumi, A. Radhakrishnan, A., Ikeda, Y., Kwon, H. J., Brown, M. S. & Goldstein, J. L. Sterol-regulated transport of SREBPs from endoplasmic reticulum to Golgi: oxysterols block transport by binding to Insig. A class of lipids with a polar head group and two non-polar tails. Kwon, H. J., Lagace, T. A., McNutt, M. C., Horton, J. D. & Deisenhofer, J. 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In agreement with Schwenk and Werthessen, a single bromination and reconversion to cholesterol was usually sufficient to establish radiochemical purity of the isolated sterol. Traffic 2, 111123 (2001). Efflux of sphingomyelin, cholesterol, and phosphatidylcholine by ABCG1. & DeBose-Boyd, R. A. Sequential actions of the AAA-ATPase valosin-containing protein (VCP)/p97 and the proteasome 19S regulatory particle in sterol-accelerated, endoplasmic reticulum (ER)-associated degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Get the most important science stories of the day, free in your inbox. Nuclear factor-B regulates the expression of multiple genes encoding liver transport proteins. A metabolite of vitamin A1 (all-trans-retinol). NUMB further recruits AP2clathrin to initiate endocytosis. J. Lipid Res. Song, B. L. & DeBose-Boyd, R. A. Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase stimulated by - and -tocotrienols. 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